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bacterial protein extraction reagent b-per  (Thermo Fisher)


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    Structured Review

    Thermo Fisher bacterial protein extraction reagent b-per
    Bacterial Protein Extraction Reagent B Per, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/b+per+bacterial+protein+extraction+reagent/b+per+bacterial+protein+extraction+reagent/med_rxiv__2025__07__10__25331115-54-0-8
    Average 90 stars, based on 1 article reviews
    bacterial protein extraction reagent b-per - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Protein Extraction:

    Article Title:
    Article Snippet: .. The resulting pellet was resuspended and lysed in B-PER Bacterial Protein Extraction Reagent (78248, Thermo Fisher) containing 50 mg/L Lysozyme (L6876, Sigma Aldrich) and 25 U/mL Benzonase (E1014, Sigma Aldrich) at a ratio of 10 mL per g of wet cell weight. ..

    Article Title: Populus PtrbHLH011 Is a Transcriptional Co-Regulator Involved in the Activation of Cell Wall Biosynthesis by Iron Deprivation.
    Article Snippet: Bacterial cells were grown in ZYM5052 autoinduction medium (VWR) at 37°C to OD600 of 0.6 and then autoinduced at 20°C overnight. .. Cells were collected and lysed using the B- PER Bacterial Protein Extraction Reagent (ThermoFisher Scientific) with lysozyme and benzonase. ..

    Article Title: Universal platform for genetic code expansion
    Article Snippet: .. Overnight expression cultures were centrifuged and resuspended in lysis buffer: B-PER Bacterial Protein Extraction Reagent (Thermo Scientific)+1X Halt Protease Inhibitor Cocktail (Thermo Scientific)+0.01% Pierce Universal Nuclease (Thermo Scientific). ..

    Article Title: VirBR counter-silences HppX3 to promote conjugation of blaNDM-IncX3 plasmids.
    Article Snippet: .. Cells were lysed in B-PER bacterial protein extraction reagent (Thermo Scientific, USA) containing 1% Triton X100, 1 mM phenylmethylsulfonyl fluoride (PMSF), 50 μg/ml lysozyme and 1 × protease inhibitors (Beyotime, China) at room temperature for 30 min on a HulaMixer. .. The cell lysates were sonicated with a Covaris M220 sonicator (75 peak power, 5% duty factor, 200 cycles/burst, 8 min duration).

    Article Title: Populus PtrbHLH011 Is a Transcriptional Co‐Regulator Involved in the Activation of Cell Wall Biosynthesis by Iron Deprivation
    Article Snippet: Bacterial cells were grown in ZYM‐5052 autoinduction medium (VWR) at 37°C to OD 600 of 0.6 and then autoinduced at 20°C overnight. .. Cells were collected and lysed using the B‐PER Bacterial Protein Extraction Reagent (ThermoFisher Scientific) with lysozyme and benzonase. ..

    Article Title: A nemaline myopathy-linked mutation inhibits the actin-regulatory functions of tropomodulin and leiomodin
    Article Snippet: .. Pellets were then resuspended according to manufacturer’s recommendations for using the B-PER® Bacterial Protein Extraction Reagent (Thermo Fisher) in the presence of 0.1 mg/mL lysozyme (Sigma) and 1x HaltTM protease inhibitor cocktail, sonicated, and spun down at 16,000 RPM (Beckman-Coulter JA-17 Rotor), 4°C, 30 min. mTmod1, mLmod2 or mLmod3 in inclusion bodies in the pellets were solubilized by resuspending pellets in 100 mM sodium phosphate pH 8.0 (pH 7.0 for mTmod1 LRR), 300 mM NaCl and 10 mM Imidazole with 8 M Urea (column buffer) overnight at 4°C on ice. .. The resuspension was centrifuged at 16,000 RPM (Beckman-Coulter JA-17 Rotor), 4°C, 30 min and the supernatant was loaded onto Ni-NTA agarose Superflow resin (Qiagen).

    Article Title: Infection-relevant conditions dictate differential versus coordinate expression of Salmonella chaperones and cochaperones.
    Article Snippet: .. The supernatant was removed, and pellets were resuspended in 50 μL of B-PER Bacterial Protein Extraction Reagent (Thermo Fisher Scientific) supplemented with 100 μg/mL lysozyme. ..

    Article Title: Infection-relevant conditions dictate differential versus coordinate expression of Salmonella chaperones and cochaperones
    Article Snippet: .. The supernatant was removed, and pellets were resuspended in 50 μL of B-PER Bacterial Protein Extraction Reagent (Thermo Fisher Scientific) supplemented with 100 μg/mL lysozyme. .. Following 5 min of lysis at room temperature, 50 μL of 2× Laemmli sample buffer was added, and the samples were boiled at 95°C for 10 min. A 5 μL portion of the mixture was electrophoresed on NuPAGE 4%–12% Bis-Tris gels (Thermo Fisher Scientific) and transferred to nitrocellulose membranes using the iBlot 2 transfer device (Thermo Fisher Scientific).

    Expressing:

    Article Title: Universal platform for genetic code expansion
    Article Snippet: .. Overnight expression cultures were centrifuged and resuspended in lysis buffer: B-PER Bacterial Protein Extraction Reagent (Thermo Scientific)+1X Halt Protease Inhibitor Cocktail (Thermo Scientific)+0.01% Pierce Universal Nuclease (Thermo Scientific). ..

    Lysis:

    Article Title: Universal platform for genetic code expansion
    Article Snippet: .. Overnight expression cultures were centrifuged and resuspended in lysis buffer: B-PER Bacterial Protein Extraction Reagent (Thermo Scientific)+1X Halt Protease Inhibitor Cocktail (Thermo Scientific)+0.01% Pierce Universal Nuclease (Thermo Scientific). ..

    Protease Inhibitor:

    Article Title: Universal platform for genetic code expansion
    Article Snippet: .. Overnight expression cultures were centrifuged and resuspended in lysis buffer: B-PER Bacterial Protein Extraction Reagent (Thermo Scientific)+1X Halt Protease Inhibitor Cocktail (Thermo Scientific)+0.01% Pierce Universal Nuclease (Thermo Scientific). ..

    Article Title: A nemaline myopathy-linked mutation inhibits the actin-regulatory functions of tropomodulin and leiomodin
    Article Snippet: .. Pellets were then resuspended according to manufacturer’s recommendations for using the B-PER® Bacterial Protein Extraction Reagent (Thermo Fisher) in the presence of 0.1 mg/mL lysozyme (Sigma) and 1x HaltTM protease inhibitor cocktail, sonicated, and spun down at 16,000 RPM (Beckman-Coulter JA-17 Rotor), 4°C, 30 min. mTmod1, mLmod2 or mLmod3 in inclusion bodies in the pellets were solubilized by resuspending pellets in 100 mM sodium phosphate pH 8.0 (pH 7.0 for mTmod1 LRR), 300 mM NaCl and 10 mM Imidazole with 8 M Urea (column buffer) overnight at 4°C on ice. .. The resuspension was centrifuged at 16,000 RPM (Beckman-Coulter JA-17 Rotor), 4°C, 30 min and the supernatant was loaded onto Ni-NTA agarose Superflow resin (Qiagen).

    Sonication:

    Article Title: A nemaline myopathy-linked mutation inhibits the actin-regulatory functions of tropomodulin and leiomodin
    Article Snippet: .. Pellets were then resuspended according to manufacturer’s recommendations for using the B-PER® Bacterial Protein Extraction Reagent (Thermo Fisher) in the presence of 0.1 mg/mL lysozyme (Sigma) and 1x HaltTM protease inhibitor cocktail, sonicated, and spun down at 16,000 RPM (Beckman-Coulter JA-17 Rotor), 4°C, 30 min. mTmod1, mLmod2 or mLmod3 in inclusion bodies in the pellets were solubilized by resuspending pellets in 100 mM sodium phosphate pH 8.0 (pH 7.0 for mTmod1 LRR), 300 mM NaCl and 10 mM Imidazole with 8 M Urea (column buffer) overnight at 4°C on ice. .. The resuspension was centrifuged at 16,000 RPM (Beckman-Coulter JA-17 Rotor), 4°C, 30 min and the supernatant was loaded onto Ni-NTA agarose Superflow resin (Qiagen).



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